[data] # directories for bpmap and CEL files # GenomeGrp: Genome Group name in bpmap file. Hs for Affy human Tiling 1.0 and 2.0 arrays # ''(blank) for Affy old Chr21/22 and Encode arrays; Mouse: Mm; Drosophila: Dm.Celegans: Ce; S.pombe: Sp # bpmap and cel files should be in the same order and have the same keys (item before =) # RepLib: full name of the the Repeat library file (optional) # Group: separates all your cel files into Treatment (1) or Control (0) groups (e.g. 111000 for 3ChIP and 3Input, 10 for 1ChIP 1Ipnut, etc) # Paired: Specify 1 if the treatment and control experiments are strictly paired, otherwise leave it blank. # If paired, the cel files should be well aligned, like: ChIP1 ChIP2 ChIP3 Ctrl1 Ctrl2 Ctrl3 BpmapFolder = /misc/iris/acct/weili/database/BPmap/Chr2122 CelFolder = /misc/iris/acct/weili/Tiling.Soft/Model.Tiling/lab/ER GenomeGrp = RepLib = /misc/iris/acct/weili/database/humanhg17_May2004/Annotation/Humanhg17Rep.lib Group = 111000 Pair = [bpmap] 1 = P1_CHIP_A.Anti-Sense.hs.NCBIv35.NR.bpmap 2 = P1_CHIP_B.Anti-Sense.hs.NCBIv35.NR.bpmap 3 = P1_CHIP_C.Anti-Sense.hs.NCBIv35.NR.bpmap [cel] 1 = MCF_ER_A1.CEL MCF_ER_A3.CEL MCF_ER_A4.CEL MCF_INP_A1.CEL MCF_INP_A3.CEL MCF_INP_A4.CEL 2 = MCF_ER_B1.CEL MCF_ER_B3.CEL MCF_ER_B4.CEL MCF_INP_B1.CEL MCF_INP_B3.CEL MCF_INP_B4.CEL 3 = MCF_ER_C1.CEL MCF_ER_C3.CEL MCF_ER_C4.CEL MCF_INP_C1.CEL MCF_INP_C3.CEL MCF_INP_C4.CEL [intensity analysis] # BandWidth: the number of bases to extended from the position being analyzed. # The result is that 2*Bandwidth probe positions are included in the signal and p-value analysis # MaxGap: maximum gap between positive probes; All regions separated by < MaxGap will be mergered into one # MinProbe: minimum number of probes for MATCh score analysis # Var: control the variance from the input (usually you need at least 3 input replicates to turn on this option) BandWidth = 300 MaxGap = 300 MinProbe = 10 Var = 0 [interval analysis] # interval cutoff can be from either MAT score or pvalue or qvalue(FDR) # if multiple cutoffs are specified, only one will be used as the real cutoff, others will be ignored. # cutoff priority: Qvalue > Pvalue > Matscore # default is Pvalue 1e-5 # Extend: extend the ChIP-enriched regions at both ends, default is 0. # set it a negative number if you want to shrink the regions. Matscore = Pvalue = 1e-5 FDR = Extend = [output] # Log: the screen output will be stored in a file if it is 1 or stderr if it is ''(blank) Log =